Table 1.

List of Primers applied in the study

Oligo name	Sequences (5’−3’)	Tm (°C)	Target	bp	Limit of detection (gn rx⁻¹)	Reference
MICf	GCCGCRAGGTGAAAMCT	60	16S rRNA inMicrocystis	248	1	Neilan et al., 1997
MICr	AATCCAAARACCTTCCTCCC	60	16S rRNA inMicrocystis	248	1	Neilan et al., 1997
mcyEcyaf	TTTGGGGTTAACTTTTTTGGGCATAGTC	56	mcyE or ndaFin Cyanobacteria	470	10	Jungblut and Neilan, 2006
mcyEcyar	AATTCTTGAGGCTGTAAATCGGGTTT	56	mcyE or ndaFin Cyanobacteria	470	10	Jungblut and Neilan, 2006
mcyAcyaf	AAAAGTGTTTTATTAGCGGCTCAT	56	mcyA in Cyanobacteria	302	10	Hisbergues, 2003
mcyAcyar	ATCCAGCAGTTGAGCAAGC	56	mcyA in Cyanobacteria	302	10	Hisbergues, 2003
mcyAmsf	ATCCAGCAGTTGAGCAA	60	mcyA inMicrocystis	171	10	Furukawa et al., 2006
mcyAmsr	GCCGATGTTTGGCTGTAAAT	60	mcyA inMicrocystis	171	10	Furukawa et al., 2006
mcyGmicf	CAACCCAACAGGTTCTTAAAGC	60	mcyG inMicrocystis	244	10	Ngwa, 2012
mcyGmicr	TGAGGCAAGGTTTCCTCTTG	60	mcyG inMicrocystis	244	10	Ngwa, 2012
nif_nostF3	ATCGTTCAACACGCAGAATTG	60	Ana, Nos, Cyl	90	10	Lu et al., 2019
nif_nostR3	TCATCCATTTCGATAGGTGTGG	60	Ana, Nos, Cyl	90	10	Lu et al., 2019
pstSf3	TGGAATGTTACCAGCAGGAATAA	60	A Flos Aq, AFA	110	10	Lu et al., 2019
pstSr3	AGTGCTGCTTGACGTAAACT	60	A Flos Aq, AFA	110	10	Lu et al., 2019