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. Author manuscript; available in PMC: 2021 Jul 8.
Published in final edited form as: Nano Lett. 2020 Jun 11;20(7):4901–4909. doi: 10.1021/acs.nanolett.0c00953

Figure 3.

Figure 3.

In vitro cell selectivity and enhanced binding avidity of the G7-aPD-L1h conjugates: (A) In vitro specificity of free aPD-L1 and the G7-aPD-L1h conjugates to PD-L1 observed using an inverted fluorescence microscope. (B-D) In vitro cell retention assay demonstrating the enhanced binding of the G7-aPD-L1h conjugates to PD-L1 expressing cells in a selective manner. An equivalent number of antibodies was immobilized on each of the aPD-L1h and G7-aPD-L1h surfaces, whereas the dendrimer-coated surface without aPD-L1 (G7-Ac-COOH) was used as a negative control. The numbers of cells remained attached to the G7-Ac-COOH-, aPD-L1h-, and G7-aPD-L1h-functionalized surfaces were compared after exposure to shear stresses of 0.36 and 3.6 dyne/cm2. All results indicate that the G7-aPD-L1h surfaces exhibit the strongest cell binding as a result of specific aPD-L1/PD-L1 adhesion. Error bars: SD.