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. 2020 Nov 4;39(24):e105594. doi: 10.15252/embj.2020105594

Figure 6. NEF impairs antibody production in HIV‐1 infected human tonsil histocultures.

Figure 6

Analysis of the impact of NEF on anti‐TT IgG production in HIV‐1 infected tonsillar blocks.

  1. Schematic overview of HIV‐1 infection and Tetanus toxoid (TT) stimulation of tonsillar histocultures.
  2. Virus replication as assessed by quantification of RT activity in the cell culture supernatant. Shown are mean values with SD from 6 donors.
  3. Production of IgG antibodies against TT from infected blocks at indicated time point. Each dot represents one donor, bar indicates mean value. Tonsillar blocks were infected with indicated virus for 24 h. After washing off the virus, blocks were stimulated with TT antigen for 24 h. Samples were collected at different time intervals to estimate viral replication and antibody production.