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. 2020 Dec 4;39(24):e106478. doi: 10.15252/embj.2020106478

Figure 3. SARS‐CoV‐2 nucleocapsid protein undergoes LLPS at physiological conditions.

Figure 3

  • A–C
    (A) Phase separation over time as monitored by turbidity of 50 μM MBP‐N after addition of TEV protease in the presence of salt (183 mM NaCl) and 0.3 mg/ml RNA at pH 7.4 (50 mM Tris), pH 6.1, pH 5.5, or pH 4.9 (pH 6.1 and below in 20 mM MES). Error bars represent standard deviation of three replicates. For samples at (B) pH 7.4 or (C) pH 5.5, DIC micrographs of 50 μM MBP‐N in the presence of salt (183 mM NaCl) without or with TEV protease (to cleave MBP from N) and without or with 0.3 mg/ml desalted total torula yeast RNA. Scale bars represent 50 μm.