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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: J Med Chem. 2020 Nov 18;63(23):14989–15012. doi: 10.1021/acs.jmedchem.0c01581

Figure 3.

Figure 3.

Concentration-response curves of RTI-13951-33, 84, and 90 in the GPR88 Lance TR-FRET cAMP assay. The TR-FRET signal (665 nm) was converted to fmol cAMP by interpolating from the standard cAMP curve. Percent inhibition of forskolin-induced (300 nM) cAMP levels was plotted against the log of compound concentration and data were fit to a three-parameter logistic curve to generate EC50 values. Each data point is the mean ± S.D. of at least three independent experiments performed in duplicate.