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. 2020 Dec 15;16(12):e9530. doi: 10.15252/msb.20209530

Figure 1. Construction of Lon and FtsH conditional mutants in Mycoplasma pneumoniae .

Figure 1

  • A–C
    RNA‐seq transcriptional profiles across the modified locus, as well as immunoblots assessing protein expression of Lon and FtsH, are shown for ΔIndLon (A), ΔIndFtsH (B), and ΔIndLon_FtsH (C) grown under inducing or depleting conditions (48 and 72 h of depletion for Lon and FtsH, respectively). Symbols +/− indicate inducing or depleting conditions. LC, loading control. WT, wild‐type. A schematic representation of the DNA rearrangements in the lon and ftsH locus is also shown for each strain. The ftsH‐inducible platform inserted by transposon delivery is shown for ΔIndFtsH and ΔIndLon_FtsH strains. The Pxyl/tetO2‐inducible promoter is highlighted with a red bent arrow and the terminator sequence used to isolate the promoter is represented by a hairpin structure. The tetR repressor gene and the resistance markers cat and tetM are indicated in purple, blue, and red, respectively.