Figure 5. Mutational analysis to identify Lon degrons in Lon substrates.

1. Amino acid sequence of the last 50 residues of FtsA (MPN316), FtsZ (MPN317), and DnaB (MPN525) proteins and their mutant derivatives. Hydrophobic motifs that can potentially act as Lon degrons are highlighted in bold. Mutations performed in these putative Lon degrons are shown in red.
2. Protein stability assessment of Lon substrates containing multiple or single mutations in putative Lon degrons. N‐terminal FLAG‐tagged derivatives with mutations shown in panel A were expressed in the ΔIndLon mutant. Protein levels were then determined by immunoblot using anti‐Lon and anti‐FLAG antibodies comparing inducing (+) or depleting (−) conditions (48 h of depletion). LC, loading control.