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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: ACS Chem Biol. 2019 Jun 17;14(7):1564–1572. doi: 10.1021/acschembio.9b00307

Figure 4.

Figure 4.

Evaluation of truncated peptide formation in pSer-3G and pSer-3.1G systems. (A) SUMO-sfGFP constructs containing N-terminal His6 purification tags and zero, one, two or three TAG sites for pSer incorporation. (B) Expression yields per liter culture of His6 -SUMO-sfGFP constructs. Error bars represent standard deviations of three expressions. (C) Coomassie stained SDS-PAGE (bottom) and corresponding Phos-tag gels (top) of purified His6-SUMO-sfGFP constructs expressed in the pSer-3G and pSer-3.1G systems. Equal quantities of fluorescent (full-length) protein were loaded in each lane.