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. 2020 Dec 9;95(1):e01582-20. doi: 10.1128/JVI.01582-20

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Copyright © 2020 Arai et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Polymerase activity of reassortant polymerases formed by subunits from ancestral and contemporary clade 2.2.1 viruses. The viral polymerase activity of reassortant polymerases formed by subunits from ancestral D1 and contemporary EG13 viruses was measured by minigenome assays in human 293T cells at 33°C and 37°C and in avian DF-1 cells at 37°C. (A to C) Polymerase activity of reassortant polymerase complexes composed of one subunit from the D1 virus and the other subunits from the EG13 virus. The data are expressed relative to the results for wild-type EG13. (D to F) Polymerase activity of reassortant polymerase complexes composed of one subunit from the EG13 virus and the other subunits from the D1 virus. The data are expressed relative to the results for wild-type D1. Each data point is the mean ± SD from three independent experiments. (G and H) Western blot analysis of reassortant polymerase complexes without (Input) and with (IP: PB2-flag) immunoprecipitation with PB2-Flag. (G) Reassortant polymerase complexes composed of one subunit from the D1 virus and the other subunits from the EG13 virus. (H) Reassortant polymerase complexes composed of one subunit from the EG13 virus and the other subunits from the D1 virus. Representative images are shown.