TABLE 1.

An overview of some of the validation tools mentioned

Software	Geometric analysis	Density fit analysis	Per‐residue analysis	Supports integration	All‐in‐one graphics	Interactive
Coot (validation menus)	Yes	Yes	Yes	Yes	No	Yes
MolProbity (web server report)	Yes	No	Yes	Yes	No	No
Polygon (comprehensive validation)	Yes	Yes	No	No	Yes	No
wwPDB (validation sliders)	Yes	Yes	No	No	Yes	No
Iris	Yes	Yes	Yes	Yes	Yes	Yes

Note: All the programs mentioned have longer run times than Iris, which are exacerbated in some cases by simple, but mandated, manual input. Coot performs all the desired analyses, but provides them in individual horizontally‐scrolled bar charts, rather than an all‐in‐one graphic. Similarly, MolProbity, which performs excellent per‐residue geometric (but not reflections‐based) analyses, provides its output as a vertically‐scrolled table. Polygon and wwPDB both provide an all‐in‐one overview for a model, but not one with residue‐by‐residue analyses.