Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 21;162(1):263–274. doi: 10.1097/j.pain.0000000000002014

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

PMC Copyright notice

Figure 1. — Nerve injury increases NICD levels in the ACC. (A) The two-way repeated-measures ANOVA revealed a significant effect of the interaction of surgery (Sham vs CCI) and time (day −1, 1, 3, 7, 10, 14, 21, and 28) on the mechanical withdrawal thresholds of rats (F = 13.76, P < 0.001***, n = 8 animals per group, two-way ANOVA). A post hoc test (Bonferroni) revealed a decrease in the mechanical withdrawal thresholds of the CCI compared to the Sham rats on day 1, 3, 7, 10, 14, 21, and 28 (black: Sham, red: CCI). (B) Two-way repeated-measures ANOVA revealed a significant effect of the interaction of surgery (Sham vs CCI) and time (day −1, 1, 3, 7, 10, 14, 21, and 28) on the paw withdrawal latency of rats (F = 12.03, P < 0.001***, n = 8 animals per group, two-way ANOVA). A post hoc test (Bonferroni) showed a decrease in the paw withdrawal latency in the CCI group compared to the Sham rats on days 1, 3, 7, 10, 14, 21, and 28 (black: Sham, red: CCI). (C–E) Western blot showing the levels of NICD in the ACC of Sham and CCI rats. Bottom panel, quantification of the intensity of the NICD bands (white: Sham group; gray: CCI group). NICD levels were only increased in the ACC at 7 days after CCI. The data are presented as mean ± SEM (P = 0.014*, Student t-test, n = 8 samples per group). On day 3 (C) and 14 (E), no difference in the NICD levels was observed. (F–H) Western blot showing NICD levels in the hippocampus of Sham and CCI rats on day 3 (F), 7 (G), and 14 (H) after surgery. Bottom panel, quantification of the intensity of the NICD bands. A difference in NICD levels in the hippocampus was not observed at different time points (Student t-test, n = 8 samples per group). ACC, anterior cingulate cortex; ANOVA, analysis of variance; CCI, chronic constriction injury; NICD, Notch intracellular domain.