Fig. 7. GATA3 and AP1 function coordinately to promote TamR-associated enhancer reprogramming and gene expression.

a, Box plots representation of gene expression in MCF7P cells. Simultaneously GATA3 KD and JUN OE (“both”) shows a more dramatic effect on the lost and gained gene expression in MCF7P cells compared to manipulating individual gene alone. P values were calculated by two-sided Wilcoxon signed-rank test. the lower and upper hinges correspond to the first and third quartiles, and the midline represents the median. The upper and lower whiskers extend from the hinge up to 1.5 * IQR (inter-quartile range). Outlier points are indicated if they extend beyond this range.

b, Heatmaps of H3K27ac ChIP-seq data at LOSS, COMMON and GAIN enhancers in MCF7P cells with the indicated treatments.

c, The aggregate plots of the normalized tag densities of H3K27ac ChIP-seq data at GAIN enhancers in MCF7P cells with indicated treatments.

d, Genome browser snapshots of H3K27ac ChIP-seq signals at the EGFR gene locus in MCF7P cells. GATA3 KD and JUN OE show a synergistic effect. The combined treatment in MCF7P cells creates an enhancer landscape similar to that in TamR cells.

e, Genome browser snapshots of GRO-seq signals at the EGFR gene locus in MCF7P cells. GATA3 KD and JUN OE (“both”) render MCF7P cells a TamR-like profile in enhancer landscape and gene expression.

f, Heatmaps of H3K27ac ChIP-seq and ATAC-seq at LOSS, COMMON and GAIN enhancers in T47D cells with the indicated treatments.

g, Genome browser snapshots of H3K27ac ChIP-seq signals at the EGFR gene locus in T47D cells. GATA3 KD and JUN OE show a synergistic effect.

h, Aggregate plots of the normalized tag densities of H3K27ac ChIP-seq data at GAIN enhancers in T47D cells with indicated treatments.

i, Western blots showing that doxycycline-induced GATA3 overexpression in TamR caused a significant decrease of interactions between endogenous ERα and JUN/RUNX2. Endogenous ERα was immunoprecipitated using anti-ERα antibody. IgG was used as a negative control.

Immunoblots are representative of two independent experiments. Unprocessed immunoblots are shown in Source Data Fig. 7.