Immunocytochemical staining of E-cadherin (A), VE-cadherin (B), TGF-β1 (C), caspase 3 (D), PARP-1 (E), and lysozyme (F) in HUVECs after 4HR treatment for 0, 8, 16, 24 hours. Noted the cytoplasmic localization (arrow heads) and nuclear localization (arrows) of different immunoreactions.