Fig 10. Ifit2 deficiency decreases CX3CR1 expression in microglia following infection.

Brain derived cells from WT and Ifit2^-/- mice either mock infected (Panel A) or infected with RSA59 (Panel B) were stained for CD45, CD11b and CX3CR1 expression at 5 days p.i. Dot plots show gating and percentages of CD45^hi CD11b⁺ and CD45^lo CD11b⁺ populations as indicated. Histograms show CX3CR1 expression on the respective gated myeloid cell populations as indicated by arrows. Panels C and D show Median Fluorescent Intensity of CX3CR1 expression by CD45^lo CD11b⁺ microglia and CD45^hi CD11b⁺ infiltrates of WT (Purple) and Ifit2^-/- (Green) mice, respectively.The experiment is repeated 2–3 times with n = 5–6. Asterix (*) represents differences that are statistically significant by Student’s unpaired t-test analysis. (***P<0.001).