Figure 1. Fabrication of a dual-crosslinked microwell hydrogel.
(a) Schematic depicting the creation of a micropatterned dual-crosslinked OMA/PEG microwell hydrogel. (b) Fluorescence photomicrograph of a micropatterned dual-crosslinked OMA/PEG microwell hydrogel with a 100 μm grid pattern. The UV blocked region (dark) consists only of a single-crosslinked network formed by Schiff base reaction between the aldehyde groups of the OMA and the amine groups of the 8-arm PEG, whereas the UV exposed regions (red) consist of dual-crosslinked networks formed by both chemical crosslinking and photocrosslinking.