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. Author manuscript; available in PMC: 2020 Dec 16.
Published in final edited form as: J Mater Chem B. 2016 Apr 18;4(20):3526–3533. doi: 10.1039/c6tb00064a

Figure 5. Osteogenic differentiation of hASC.

Figure 5.

(a) Alizarin red S stained images of hASCs in traditional two-dimensional culture on tissue culture plastic (2D), conventional suspension culture in a spinner flask (Suspension), and suspension culture of spheroids in hydrogel microwells a spinner flask (Microwell). The scale bars indicate 2 mm. (b) Quantification of calcium content normalized to the DNA. *p<0.05 compared to 2D at specific time point. Size distribution of hASC multicellular spheroids in the (c) Suspension and (d) Microwell groups.