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Homomultimerization of S, S1 and S2 on the virus particles. Urea (6M) was added in loading buffer containing 2% NP-40 to dissociate S multimers and proteins were separated by 8% of gradient SDS-PAGE. Rabbit α-S2 was used in WB. Molecular weight markers are indicated on the left in kilodaltons and proteins are indicated by the arrows on the right. The experiment was repeated 3 times at least.
