Figure 6.

Multi-bands of S1 and time course of viral protein modifications. (A) Purified virions were analysed by 4–12% SDS-PAGE at 20mA, and transferred at 380 mA for 70 min. (B) Supernatants of infected cells were collected on days 1 to 4 post-infection and analysed by WB using rabbit-α-virion serum. The proteins were separated by 4-20% of gradient SDS-PAGE and transferred to Nitrocellulose membrane. S′ and M′, unglycosylated; 3a′ cleaved. Molecular markers are indicated on the left in kilodaltons and proteins are indicated by the arrows on the right. The experiments were repeated 3 times at least.