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. 2020 Dec 14;9(1):2642–2652. doi: 10.1080/22221751.2020.1854623

Figure 5.

Figure 5.

Culex pipiens pre-exposed to USUV infectious blood meal show a decreased WNV infection and transmission rate. (A) Schematic overview of the sequential blood meal experiment design. (B) Bar graph shows the percent of WNV positive mosquito bodies and saliva of the total engorged mosquitoes at 14 days after the WNV blood meal. “C”, “U” and “W” represent virus-free, USUV and WNV infectious blood meal, respectively. (+) and (n) indicate the numbers of WNV positive mosquito bodies/saliva and the total numbers of the engorged mosquitoes, respectively. The results present cumulative numbers from four independent experiments (Table S5). Fisher’s exact test was performed on the cumulative data. Asterisks (*) and (***) indicate significance at P < 0.05 and < 0.001, respectively. (C) WNV genome copies in mosquito bodies and (D) saliva after a sequential blood meal exposure. Kruskal-Wallis with Dunn's multiple comparison or t test was used to compare the mean of the genome copies among three or two data sets, respectively. ns indicates no significant difference. Black dash lines represent the cut-off value for WNV genome copies which corresponds to a Ct value of 34.