Loading of different types of payloads into nanobottles. (A, B) Fluorescence images of PS nanobottles loaded with (A) coumarin-6 and (B) DNP-BSA (C, D) TEM images of PS nnoabottles loaded with aqueous ioversol solutions at concentrations of (C) 25% and (D) 51%. (E) SEM and TEM (insets) images of PS nanobottles loaded with iron oxide nanoparticles. (F) SEM image of PS bottles loaded with PS solid nanoparticles. For the PS bottles in (A-D) and the left inset in (E), their openings were closed by heating the system at 95 °C for 30 min. (A, B, E, F) Reproduced with permission.[7] Copyright 2005 Springer Nature. (C, D) Reproduced with permission.[45] Copyright 2012 Wiley-VCH.