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(A) Assessment of mineralization by Alizarin red S (ARS) staining of the cell seeded on spiral, porous cylinder, and porous spiral scaffolds for 14, 21, and 28 days; (B) The standard curve of ARS in cetylpyridinium chloride for quantification; (C) Calcium deposition quantified by ARS assay extracted from day 14, 21 and 28 (*: P < 0.05).
