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Simulated mass spectra of (a) a hypothetical 2 MDa (50 kDa FWHM) analyte particle with 70 to 80 charges, (b) selection (900 FHWM) of most abundant charge states, (c) up to 50 proton transfer reactions of the selected analyte charge states, and (d) up to 5 attachments (indicated by colored numbers) of the 10− charge states holomyoglobin (17,567 Da) to the selected analyte charge states. Abundance scales in (c) and (d) are relative to (b).
