Figure 4.
Ability of P. gingivalis to escape antibiotic and serum killing effects. (A) Porphyromonas gingivalis was separated and coated on brain heart infusion (BHI) plates. For the assay, 6-mm diameter cefazolin (30 μg/tablet) and sulfamethoxazole (300 μg/tablet) drug-sensitive paper was placed on BHI plates for 7 days. The black dotted line indicates the inhibition zone. (B) The diameters of the inhibition zone were measured and compared. (C) After co-culturing with serum for 1 h, the survival rates was compared. Viable counts were based on CFU. The calculation formula is as follows: survival rate (%) = (number of bacteria surviving after fresh serum co-incubation/number of bacteria surviving after inactivated serum co-incubation) × 100%. *p < 0.05; **p < 0.01; ***p < 0.001; NS p > 0.05. LH, low heme; HH, high heme; Coc, coculture; and Mon, monoculture.