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. 2020 Dec 15;10:21969. doi: 10.1038/s41598-020-77714-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-125b-2 increased expression levels of fat synthesis-associated genes. (A–C) Expression levels of C/EBPα, PPARγ, and SCD-1 mRNA in WT and KO mice fed a ND or HFD. SCD-1, PPARγ, and C/EBPα were significantly increased in KO mice fed a HFD. (D,E) Expression levels of C/EBPα, PPARγ and SCD-1 protein in WT and KO mice fed a ND. (F,G) Expression levels of C/EBPα, PPARγ and SCD-1 protein in WT and KO mice fed a HFD. C/EBPα, PPARγ, and SCD-1 protein expression was significantly increased in KO mice fed a HFD. The images displayed in Figures D and F are cropped blots, the full-length blots of (D,F) are presented in Supplementary Fig. 2. Data are presented as mean ± SE (*P < 0.05, ** P < 0.01, n = 8 mice per group).