Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 15;11:6389. doi: 10.1038/s41467-020-19929-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Left: 16S rRNA gene-based phylogenetic tree showing the diversity of isolates able to deconjugate bile acids. Species (corresponding branches) are coloured according to phyla as in Fig. 1. Right: magnified pictures of a culture of Fusobacterium mortiferum grown on plates (WCA, Wilkins–Chalgren-Anaerobe medium) without (top) or with (bottom) addition of 0.5% (w/v) tauro-conjugated deoxycholic acid (TDCA). Two criteria were considered as indicative for positive reaction: (i) whitening of the colonies; (ii) formation of a halo surrounding colonies (solid medium; white arrows in the picture) or precipitates visible at the bottom of the wells and jellification of the medium when grown as liquid cultures (not shown). The scale bars represent 5 mm. b Chromatograms showing the conversion of cholic acid to deoxycholic acid by C. scindens DSM 100975. KDCA, ketodeoxycholci acid. The two chromatograms on top span the entire run time. The bottom ones are zoom-in sections (XIC:+MRM m/z 424.367/371.300 Da) until the indicated time to visualize the appearance of 3-KDCA. c Venn diagram of comparative genomic analysis between the human faecal isolate C. scindens ATCC 35704 and the pig isolate DSM 100975 (=BL-389-WT-3D). SC single-copy, PCG protein-coding genes, OG orthologous group. d Gene organization of the bile acid-inducible (bai) genes in the two strains and overview of the bile acid 7-alpha-dehydroxylation biochemical pathway. e Scatterplots of average log₂(CPM) vs. log₂(fold-change) from duplicate 0.1 mM cholic acid (CA) or deoxycholic acid (DCA) induced cultures of C. scindens DSM 100975. Genes involved in bile acid metabolism are labelled. f Heat map of bile acid-metabolizing genes from CA- and DCA-induced transcriptomes. Genes included in e and f were differentially expressed (+/−) 0.58 log₂(FC) with false discovery rate (FDR) < 0.05.