Table 1.
Peak | Retention time (min) | Identification | Day 0 | Day 7 | ||
---|---|---|---|---|---|---|
UV− | UV+ | UV− | UV+ | |||
1c | 19.5–20.5 | Gallic acid | 2.9 a | 1.3 d | 2.3 b | 1.5 c |
1d | 21.3–22.5 | Coumaroyl quinic acid | 4.0 c | 5.3 b | 3.9 c | 8.1 a |
1e | 30.25 | Cyanidin 3-rhamnoside | 1152 a | 416 b | 47 d | 276 c |
2 | 33.03 | Pelargonidin 3-rhamnoside | 155 a | 60 b | 25 c | 52 b |
3 | 37.37 | Pelargonidin | 4 b | 7 a | 0.37 d | 1.6 c |
4 | 41.32 | Peonidin-3-xylopyranoside | 28 b | 156 a | ND | 6 c |
5 | 46.19 | Cyanidin 3-rutinoside | 12 c | 67 a | ND | 19 b |
6 | 47.04 | Kaempferol 3-O-robinobioside-7-O-arabinofuranoside | 3 c | 1.47 d | 5.5 a | 3 b |
7 | 53.18 | Isorhamnetin 3-O-galactoside | 3 a | 3 a | ND | 2.7 b |
8 | 56.06 | Isorhamnetin 3-O-glucoside | 17 d | 21 c | 37 b | 43 a |
9 | 57.16 | Isorhamnetin | 1.2 b | 1.1 c | 1.3 a | ND |
10 | 60.4 | Kaempferol 7-O-neohesperidoside | 5 b | 3.3 c | 3.1 d | 7 a |
11 | 65.88 | Kaempferol 3-O-arabinofuranoside | 10.5 d | 13.8 c | 17.6 b | 18.1 a |
12 | 67.19 | Quercetin 3-O-glucoside | 2.8 b | 2.8 b | 0.5 c | 4.7 a |
13 | 70.38 | Quercitrin-2″-O-gallate | 1.1 b | 0.8 d | 1.9 a | 1.0 c |
14 | 73.88 | Myricetin-3-O-glucuronide | 0.1 b | 14.3 a | ND | ND |
15 | 74.36 | Myricetin-3-O-glucoside | 16.1 b | 0.1 c | 0.25 c | 25.1 a |
16 | 76–77.85 | Myricetin rhamnoside | 107 c | 74 d | 196 a | 167 b |
Values represent the mean of three replicates. Values with different letters within the same compound represent statistical difference (p < 0.05) by the Tukey’s HSD test. Concentration values for each type of compound were determined from chromatogram peak areas and standards (Table S1). Areas of peaks 1e to 5 were quantified at 520 nm (Fraction F4) and areas of peaks 1c, 1d, 6–16 were quantified at 330 nm (Fraction F3).
ND not detected.