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. 2020 Dec 15;10:22011. doi: 10.1038/s41598-020-78886-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TREK-1 activation causes plasma membrane potential (Em) hyperpolarization: Representative curves of Em-sensitive FLIPR assays showing that TREK-1 activation with BL1249 causes Em hyperpolarization in primary mouse AT2 cells under both room air and HO conditions (A,B), as indicated by a decrease in fluorescence values. Red arrows on the Y-axis indicate relative fluorescence values reflective of the baseline Em value in room air and HO exposed AT2 cells, demonstrating that HO exposure itself causes Em depolarization (higher baseline fluorescence value in B than A; *BL1249 compared to no drug/vehicle control, p ≤ 0.05). (C) Summary of baseline Em values of RA- vs. HO-exposed AT2 cells averaging n = 6 independent experiments for each condition. Data are represented as Box-Whisker plots with medians, 1st and 3rd quartiles, and max and min values; ^compared to room air exposed AT2 cells; p ≤ 0.05, individual experiments were run in triplicates.