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. 2020 Dec 15;10:21967. doi: 10.1038/s41598-020-78955-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Acidosis can decrease the glycolysis in cancer cells. Glucose consumption (a) and lactate excretion (b) of breast cancer cells under different culture conditions. ¹³C NMR spectra of labeled lactic acid in MCF-7 and MDA-MB-231 cell lines (c). The MCF7 (d) and MDA-MB-231 (e) cells were detected for ECAR after treated for 48 h. Values are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired Student’s test, n ≥ 6.