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Pre-study to find an efficient concentration of heparin. (A) HK-2 cells treated with 40 U histone were administered with different concentrations of heparin and cultured for 6 h. Flow cytometry was used to evaluate apoptosis rate. (B) The proportion of apoptotic cells was significantly lower at doses of 25 and 37.5 IU/ml in comparison to 12.5 IU/ml (P < 0.05). Data are presented as mean ± SD, n = 3 per group of the representative data from three independent experiments; *P < 0.05.
