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. 2020 Dec 15;11(12):1075. doi: 10.1038/s41419-020-03289-w

Fig. 5. Ndufs6−/−-BM-MSCs exhibit complex I deficiency and enhanced ROS generation.

Fig. 5

A The protein level of complexes I, II, III, and IV was evaluated in BM-MSCs and Ndufs6−/−-BM-MSCs. B The intracellular ROS of BM-MSCs and Ndufs6−/−-BM-MSCs was measured by DCFH-DA staining. C The intracellular ROS of BM-MSCs and Ndufs6−/−-BM-MSCs was statistically quantified. D The mitochondrial ROS of BM-MSCs and Ndufs6−/−-BM-MSCs was measured by Mito-Sox staining. E The mitochondrial ROS of BM-MSCs and Ndufs6−/−-BM-MSCs was statistically quantified. F The MMP of BM-MSCs and Ndufs6−/−-BM-MSCs was assessed by TMRM staining. G The MMP of BM-MSCs and Ndufs6−/−-BM-MSCs was statistically quantified. H NR treatment decreased cellular senescence in Ndufs6−/−-BM-MSCs. Results are presented as mean ± SD. n = 3. **p < 0.01.