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. 2020 Dec 15;3:774. doi: 10.1038/s42003-020-01514-y

Fig. 5. In vitro culture expansion of human mesenchymal stem cells (hMSCs) alters NAD+ biogenesis and metabolism.

Fig. 5

a mRNA levels of NAD+ metabolic enzymes NAMPT, CD38, and CD73 in hMSCs at early and late passage. b NAMPT, c CD38, and d CD73 protein expressions were all increased in late passage of hMSCs determined by flow cytometry. NG negative control. e, f Western blot confirmed the increase of NAMPT, CD38, and CD73 in late passage of hMSCs. Biological replicates (n): n = 3. *p < 0.05. NS not statistically significant.