Table 1.
Bacterial strains used in this study
| Organism | Serovar | Reference/field strain | Detection results | |
|---|---|---|---|---|
| Real‐time RPA | Real‐time PCR | |||
| Haemophilus parasuis | 1 | NO.4 | + | + |
| 2 | SW140 | + | + | |
| 3 | SW114 | + | + | |
| 4 | CVCC3894 | + | + | |
| SW124 | + | + | ||
| 5 | CVCC3895 | + | + | |
| Nagasaki | + | + | ||
| 6 | 131 | + | + | |
| 7 | 174 | + | + | |
| 8 | C5 | + | + | |
| 9 | D74 | + | + | |
| 10 | H367 | + | + | |
| 11 | H465 | + | + | |
| 12 | H425 | + | + | |
| 13 | 17,975 | + | + | |
| 14 | 22,113 | + | + | |
| 15 | 15,995 | + | + | |
| ND | Field strain | + | + | |
| ND | Field strain | + | + | |
| ND | Field strain | + | + | |
| Actinobacillus pleuropneumoniae | 1 | CVCC259 | − | − |
| 3 | CVCC261 | − | − | |
| 5 | CVCC263 | − | − | |
| 7 | CVCC265 | − | − | |
| 8 | CVCC266 | − | − | |
| Pseudomonas aeruginosa | CICC21636 | − | − | |
| Field strain | − | − | ||
| Streptococcus hemolytic‐β | CMCC10373 | − | − | |
| Bacillus cereus | ATCC 14579 | − | − | |
| Field strain | − | − | ||
| Pasteurella multocida | Field strain | − | − | |
| Field strain | − | − | ||
| Bordetella bronchiseptica | Field strain | − | − | |
| Mycoplasma hyopneumoniae | Strain 168 | − | − | |
| Klebsiella pneumoniae | ATCC 4352 | ‐ | ‐ | |
| Field strain | ‐ | ‐ | ||
| Mannheimia haemolytwa | Field strain | ‐ | ‐ | |
| Listeria monocytogenes | ATCC 15313 | − | − | |
| Field strain | − | − | ||
| Staphylococcus aureus | ATCC 6538 | − | − | |
| Field strain | − | − | ||
Abbreviations: −, negative; +, positive; ATCC, America Type Culture Collection; CICC, China Center of Industrial Culture Collection; CMCC, National Center for Medical Culture Collection; CVCC, China Veterinary Culture Collection Center; ND, not determined.