TABLE 2.
Effect of selected disinfectants on ASF virus detection via qPCR assay
| Disinfectant group and identity | Final dilution (w/v) | ASF titre following addition of disinfectant | |||||
|---|---|---|---|---|---|---|---|
| 1 min | p‐value | 15 min | p‐value | 30 min | p‐value | ||
| Quaternary ammonium and glutaraldehyde mixture (omnicide) | 1:50 | 6.63 ± 0.05 | >0.05 | 6.30 ± 0.01 | <0.001 | 5.96 ± 0.02 | <0.001 |
| 1:150 | 6.66 ± 0.01 | >0.05 | 6.33 ± 0.16 | >0.05 | 5.88 ± 0.14 | <0.05 | |
| 1:300 | 6.67 ± 0.02 | >0.05 | 6.36 ± 0.22 | >0.05 | 5.97 ± 0.04 | <0.01 | |
| Sodium hydroxide 2% solution (caustic soda) | 1:25 | ND | <0.001 | ND | <0.001 | ND | <0.001 |
| 1:50 | ND | <0.001 | ND | <0.001 | ND | <0.001 | |
| 1:100 | 5.35 ± 0.51 | >0.05 | 3.97 ± 0.69 | <0.05 | ND | <0.001 | |
| Chlorine 5% solution (84 product) | 1:100 | ND | <0.001 | ND | <0.001 | ND | <0.001 |
| 1:200 | 5.42 ± 0.65 | >0.05 | ND | <0.001 | ND | <0.001 | |
| 1:300 | 4.69 ± 0.84 | >0.05 | 4.07 ± 0.62 | <0.05 | ND | <0.001 | |
| Sodium hypochlorite 5% solution (bleach) | 1:250 | 5.78 ± 0.47 | >0.05 | 5.19 ± 0.18 | <0.01 | ND | <0.001 |
| 1:500 | 6.48 ± 0.08 | >0.05 | 5.79 ± 0.21 | <0.05 | ND | <0.001 | |
| 1:1,000 | 6.50 ± 0.17 | >0.05 | 6.43 ± 0.15 | >0.05 | 6.02 ± 0.12 | <0.05 | |
African swine fever (ASF) virus culture by standard methods (OIE, 2019b; Zhao et al., 2019). Starting amount of ASF virus added to each reaction: 4.6 × 106 copies, measured by TCID assay. Data presented as log mean of ASF titre in qPCR assay (Fernández‐Pinero et al., 2013) ±SD. p Value data derived by one‐way analysis of variance, using SPASS software. ND indicates ASF virus DNA below the level of detection in qPCR assay used (Fernández‐Pinero et al., 2013).