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. 2021 Jan 1;12(1):232–243. doi: 10.7150/jca.49560

Figure 2.

Figure 2

Proliferation ability and tumor formation decreased in prostate cancer cells after knocking down ACACA gene in vitro or in vivo. (A) Western blot detected the effect of the sh-ACACA-c vector transfected in DU145 and PC3 cells. (B) Cytometry flow showed that ACACA knockdown resulted in an extension of G1 phase of cell cycle in DU145 and PC3 cells. (C) CCK-8 assay showed the ability of proliferation in prostate cancer cells after knocking down the ACACA gene. (D) Inhibition of ACACA gene in DU145 cells significantly retarded subcutaneous tumor growth in nude mice (n=11). (E) Hematoxylin and eosin (H&E) and Immunohistochemistry (IHC) staining of tumor tissue. (F-G) The tumor volume curve and weight were shown. Data were presented as Mean ± SD. *P < 0.05. **P < 0.01.