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. 2021 Jan 1;12(1):253–263. doi: 10.7150/jca.49709

Figure 2.

Figure 2

Knockdown of NKCC1 inhibited the proliferation, migration and invasion of GC cells. A-B. NKCC1 protein and mRNA levels were observed in the GC cell lines and GES-1 cells. C. Representative immunofluorescence images showing localization of NKCC1 on the plasma membrane of the GC cells (magnification - 200X, Scale bars: 50 µm). D-G. NKCC1 mRNA and protein levels in GC cells transfected with shNKCC1 and scrambled control. H-I. The proliferation capacities were detected by CCK8 assays in GC cells transfected with shNKCC1 and scrambled control. J-K. Representative images of wound healing assay showing the in vitro migration of control and shNKCC1 GC cells (magnification - 40X, Scale bars: 200 µm). L-M. Transwell assay showing in vitro migration and invasion of control and shNKCC1 GC cells (magnification - 100X, Scale bars: 100 µm). * P < 0.05; ** P < 0.01; *** P < 0.001.