AMPK inhibitor decreased the levels of SIRT1, cellular apoptosis and oxidative stress at 24 h after SAH, which inversely stimulated the activation of AMPK. (A, B) Representative western blot images and quantitative analyses of p-AMPK and SIRT1; (C, D) Representative western blot images and quantitative analyses of p-AMPK, SIRT1, PGC-1α, GPx, SOD. n = 6 for each group; (E) Levels of ROS at 24 h after SAH. n = 6 for each group. Data were shown as means ± SD, and were compared using 1-way ANOVA followed by the Tukey post hoc test. *p < 0.05 vs. sham, &p < 0.05 vs. SAH + vehicle, #p < 0.05 vs. SAH + BMS-470539.