Figure 1.

pFUS increases TUNEL reactivity without apoptosis in tumor cells. A) Representative imaging (TUNEL-positive nuclei in red) and B) quantification of TUNEL reactivity in tumor cells with or without pFUS (n = 9 per group per cell line) at 6 h post-pFUS. C) Representative imaging of ICC for cleaved (activated) caspase-3 (green) in tumor cells with or without pFUS at 6 h post-pFUS. D) Quantification of activated caspase-3 in each group (n = 9 per group per cell line). H₂O₂ group in each panel represents a positive controls for each measurement where cells were incubated with H₂O₂ (1 mM) for 6 h. Asterisks represent p < 0.05 by ANOVA comparisons performed on all groups for each cell line. Scale bars = 50 μm.