Figure 3.

pFUS increases Ca²⁺-dependent superoxide formation in cells, but neutralization of superoxide by SOD mimetic does not reduce pFUS-induced increases in TUNEL reactivity. A) Representative imaging of Mitosox fluorescence intensity (red; scale bars = 100 μm) and B) quantification with or without pFUS and in the presence or absence of intracellular BAPTA at 2 h post-sonication (n = 15 per group per cell line). Mitosox was loaded immediately prior to pFUS. Asterisks represent p < 0.05 from ANOVA comparisons performed on all groups from each cell line. C) Quantification of TUNEL reactivity with or without pFUS following incubation with the SOD mimetic mtTEMPOL (n = 9 per group per cell line). Asterisks represent p < 0.05 by t-tests between treated and control groups for each cell line.