Figure 2.

Antitumor effects of W2014-S and W2014-R against malignant cells harboring aberrantly-active STAT3 in vitro. NSCLC cell lines A549 and PC-9 with constitutive STAT3 were treated with W2014-S or W2014-R at different concentrations (0, 0.3, 1, 3 μM). (A) Cell proliferation and IC50 values were measured in A549 and PC-9 cells at 72 h. Each point represented the mean ± SEM, n=3. (B-C) Numbers of colonies of A549 and PC-9 cells were detected by colony formation assay. Data were expressed as mean ± SEM, n=3. ^*P < 0.05, ^**P < 0.01, *^**P < 0.001. (D-E) Cell migration of A549 cells was detected by wound healing assay. Data were expressed as mean ± SEM, n=3. ^*P < 0.05, ^**P < 0.01, *^**P < 0.001. (F-G) Cell invasion of A549 cells was measured by transwell cell invasion assay. (H-I) A549 and PC-9 cells were treated with W2014-S for 72 h and apoptosis was measured by flow cytometry. The figures were modified due to the lower font size of labels in original picture. PI means propidium iodide. Data were expressed as mean ± SEM, n=3. ^*P < 0.05, ^**P < 0.01, *^**P < 0.001. (J) Western bolt was used to detect the expression of c-Caspase-7 protein.