Figure 1.

SNI causes a persistent increase in circulating classical monocytes. (A) Representative flow cytometry profiles of different subtypes of leukocytes gated by CD45⁺ in sham (Sh) and SNI mice. The inserted number shows the percentages of the classical monocytes (CMo: CD45⁺CD11b⁺L6C^high, red), CD45⁺CD11b⁺L6C^low cells (M, blue), granulocytes (G: CD45⁺CD11b⁺L6C^med, purple) and lymphocytes (L: CD45⁺CD11b^-, golden) in all CD45⁺ leukocytes. Sh: sham group, S1d, S3d, S9d, S14d, S21d show the groups harvested at different days after SNI. (B, C) Percentages of blood classical monocytes (CMo), CD45⁺CD11b⁺L6C^low cells (M), granulocytes (G) and lymphocytes (L) in all leukocytes (CD45⁺) from different groups. n = 10 for naïve and S9d groups. In sham mice, the experiments were performed at three time points (1 d, 3 d, 9 d after sham operation, 3 mice at each time point). As no difference was detected, the data from sham mice were used together; n = 4 in other groups. (D-F) Changes in nonclassical monocytes (NCMo: CD45⁺CD11b⁺Ly6C^lowCD49b^-, C), NK cells (CD45⁺CD3^-CD49b⁺, D), and neutrophils (CD45⁺SSC^medLy6G⁺, F) in sham and SNI groups (n = 3-5 mice/group). *P < 0.05, **P < 0.01, ***P < 0.001, and n.s.: not significant, vs. sham group, two-way ANOVA with Bonferroni's post hoc test used for B and C, one-way ANOVA with Bonferroni's post hoc test used for D-F.