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. 2021 Jan 1;11(3):1059–1078. doi: 10.7150/thno.44364

Figure 4.

Figure 4

SNI causes progressive gliosis in many brain regions, especially in bilateral hippocampi. (A, B) Temporal change of the morphology, normalized integrated density (IntDen) of CD11b and GFAP, and numbers in microglia and astrocytes in bilateral hippocampi at 1, 3, and 9 d after SNI. (C-E) Fluorescent IntDen of CD11b and GFAP in different brain regions as indicated in sham and SNI groups at 9 d after surgery. CD11b- and GFAP- immunostaining micrographs in both groups are from the same section. The white line boxed areas in C are magnified in A to show the morphological changes of glial cells. The images in D are magnified from dotted boxes in C. RawIntDen analysis of CD11b and GFAP staining was used for statistics in E. Hip: hippocampus, S1: primary somatosensory cortex, RSGc: retrosplenial granular cortex, Thal: Thalamus, Hypo: hypothalamus, Amy: amygdala. Scale bars: 100 µm (A), 500 µm (C) and 25 µm (D). n = 3 mice/group, 3-4 images/mice, *P < 0.05, **P < 0.001, ***P < 0.001 vs. sham group, two-way ANOVA with Bonferroni's post hoc test.