Figure 1.

Pharmacological inhibition of NHE1 in combination with TMZ increase immune cell infiltration determined by ex vivo¹H/¹⁹F MRI and immunostaining. (A) Experimental protocol showing that SB28-GFP cell implanted mice at 5 d.p.i. received either vehicle (PBS-DMSO), HOE642 (H), TMZ (T), or T+H combination treatments. VS1000H DM red emulsion was injected via tail vein on 16 d.p.i. and at 18 d.p.i mice were sacrificed to perform ex vivo MRI. (B-C) Representative images of ¹H/¹⁹F MRI tracking for GAMs. ¹⁹F MRI (pseudocolor, arrowheads) over greyscale anatomical image (arrow). Immune cell infiltration index was computed as ¹⁹F signal divided by tumor volume and data were normalized to the control. (D) Representative confocal images of perfluorocarbon (VS1000H, Texas Red, arrowheads) loaded GAMs and IBA1 staining of brain sections collected post-MRI from the same cohort of mice used in panel (A-C). (E) Summary data of IBA-1 cells loaded with VS1000H dye. (F) Summary data of IBA-1⁺ cells infiltrated in the tumor core. Data are mean ± SEM, n = 5-7 mice/group. *p < 0.05 vs indicated.