Figure 3.

Pharmacological inhibition of NHE1 in combination with TMZ increase glucose uptake and mitochondrial respiratory function in SB28-GFP tumor-infiltrating GAMs. (A) Experimental protocol showing that SB28-GFP cell implanted mice at 5 d.p.i. received either vehicle (PBS-DMSO), HOE642 (H), TMZ (T), or T+H combination treatments. Flow cytometric analysis or Seahorse XFe96 analysis was subsequently performed. (B-F) Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured in tumor-infiltrated CD11b⁺ cells. (G-H) 2-NBDG uptake and mitochondrial Mitotracker staining of tumor infiltrated microglia (CD11b⁺/CD45^low-medium) and myeloid (CD11b⁺/CD45^high) cells. (I-J) 2-NBDG uptake and Mitotracker staining of tumor-infiltrated CD8⁺ and CD4⁺ T-cells. Data are mean ± SEM. n = 5 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001 vs indicated.