Figure 5.

Effect of pharmacological inhibition of ASK1 or p38 on AA-induced HSP27 phosphorylation. (A) Immunoblot of MDMs pretreated with the selective ASK1 inhibitor Selonsertib (1 µM) or the p38 inhibitors BIRB796 or SB203580 (10 µM) for 1 h prior to treatment with AA for 30 min. (B) Quantification of n = 7 biological replicates. Data points represent individual samples, horizontal lines indicate the median. Statistical analysis was performed by paired t test. **** adjusted p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05 against samples treated with AA + solvent. Detection of pp38 was not reliably possible in solvent only samples. ns: not significant. Values represent the fold change relative to AA + solvent.