Figure 9.
Effect of AA on actin filament organization and macropinocytosis. (A) Phalloidin staining of actin filaments in MDMs treated with 50 µM AA or solvent (control) for 24 hrs (representative experiment). (B) Quantification of actin filament rearrangement in MDMs from n = 6 donors after treatment as in panel A (each donor is represented by a specific symbol). Cells were counted as positive for actin rearrangement, if the typically wavy actin fibers were undetectable and/or actin filaments were accumulated at the cell edges. For each sample, on average 70 cells were evaluated. (C, D) Flow cytometric analysis of FITC-Dextran pinocytosis by MDMs treated with 50 µM AA for 24 h. Latrunculin A was included as a known pinocytosis inhibitor 67. Untreated MDMs incubated on ice to invoke a complete inhibition of pinocytosis were used as negative control. Significance was tested by paired t test: ****p < 0.0001, ***p < 0.001, * p < 0.05. The gating strategy is shown in Fig. S6.