Figure 8.

NLN-KLA and NEW-KLA inhibit the primary tumor growth and metastasis in 4T1 spontaneous breast tumor metastasis model. (A) Treatment protocols. NLN-KLA, NEW-KLA, combination of NLN and KLA (NLN + KLA), or combination of NEW and KLA (NEW + KLA) was intravenously injected into BALB/c mice bearing 4T1 tumor at mammary fat pads at the indicated time points (10 mg/kg body weight, thrice per week for three weeks). (B) Whole-body bioluminescence imaging to monitor tumor progression. (C) Quantification of the total photon flux (number of photons/second) in the whole body. (D) Tumor volumes. (E) Tumor weights. (F) Numbers of metastatic tumor nodules in the lungs. (G) Percent survival. (H) TUNEL staining (red) in primary tumor tissues. Nuclei were stained with DAPI (blue). Scale bars = 20 µm. (I) Body weights. Data are mean ± S.E. (n = 10 per group). *, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s, not significant compared with phosphate-buffered saline (PBS) by one-way ANOVA.