Figure 1.

Light-sheet fluorescence microscopy (LSFM) to uncover the 3-D microvascular network. (A) A schematic illustration of the intact ocular globe, consisting of retinal and choroidal vasculature that were imaged and analyzed. (B) A superficial primary retinal vascular plexus lies in the nerve fiber layer (NFL), whereas the secondary plexus is located deep in the outer plexiform layer (OPL). (C) The pipeline to quantitatively analyze the 3-D hemispherical retinal vascular plexus using a optimized CLARITY method and LSFM. (D) An optimized passive CLARITY method was applied to optically clear the retina as articulated in the Methods section.