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. 2021 Jan 1;11(3):1162–1175. doi: 10.7150/thno.53073

Figure 2.

Figure 2

LSFM imaging of the unscathed 3-D hemispherical retina. (A-B) The unscathed vasculature in the 3-D retina was used to define representative regions of interest. The white dashed line depicts the contour of the hemispherical retina. (C) The vertical sprouts, bridging the primary and secondary plexus, are highlighted in the representative 2-D section (red dashed lines in B) of the retinal vascular network. (D) 3-D vertical sprouts between the primary and secondary vascular plexus (blue box in B) are located in the nerve fiber layer and the outer plexiform layer. (E) The 3-D and (F) 2-D peripheral regions (orange box in B) of the retina are enlarged. (G) The maximum intensity projection and (H) depth color-coded images of the capillary network (dashed box in F). Scale bars: (A-C) 500 µm; (D) 100 µm; (E-F) 300 µm; (G-H) 50 µm. Depth color-coded scale: 0~240 µm in (H).