Figure 7.

Quantitative analysis of the volumes for the vertical sprouts and plexuses in P12 normoxia and OIR mice. (A-B) Severe vaso-obliteration and depletion of vertical sprouts (in blue) and the secondary plexus (in yellow) were demonstrated in the 3-D rendering of the OIR group. (C-D) The quantification of the volume fraction of both sprouts and plexuses from both middle and peripheral regions indicate significant vessel depletion in the OIR group (*p < 0.05 vs. normoxia, by unpaired two-tailed Student's t-test, n = 5 per group). (E) The surface plot of the dice coefficients on different sizes of sliding windows and values of cutoff angles. (F) The representative VOI demonstrating the orientation color-coded 3-D rendering of the vascular network (X: Blue, Y: Green: Z: Red). Scale bar: 200 µm for A-B and 100 µm for F.