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. 2021 Jan 1;11(3):1079–1099. doi: 10.7150/thno.49354

Figure 2.

Figure 2

The influence of miR-802 expression on lipid metabolism in murine hepatocytes is AMPK dependent. (A) qRT-PCR analysis of gene expression related to lipo-metabolism in FL83B cells with miR-802 mimic or inhibitor treatment. (B, C) qRT-PCR analysis of Prkaa1 and Prkaa2 expression in FL83B cells upon transfection with miR-802 mimic or inhibitor. (D, E) Western blot analysis of protein levels of Prkab1, Prkaa1, Prkaa2 upon transfection with miR-802 mimic or inhibitor in FL83B cells. (F) Western blot analysis of phosphorylated AMPK and ACC in FL83B cells after stimulating with A769662, miR-802 mimic or A769662 + miR-802 mimic. (G) Western blot analysis of phosphorylated AMPK and ACC in FL83B cells after stimulating with miR-802 inhibitor, compound C or miR-802 inhibitor + compound C. (H) ACC activity in FL83B cells after treating with miR-802 mimic or inhibitor. Data are expressed as the mean ± s.e.m of 3 independent experiments with similar results. *P <0.05, **P <0.01, ***P <0.001.